| Gene | Pck2 |
| Chromosome | 14 |
| Deletion Start | 55,548,778 |
| Deletion End | 55,540,407 |
| Deletion Size | 8,372 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 55,540,563 | 55,540,582 | TCCTCCGCTTGTTTCCCATC |
| TUP | 55,540,588 | 55,540,617 | AAGAGACTGGCAGGGTGGCTATTAATCAGT |
| TUR | 55,540,621 | 55,540,638 | ATGCACCACCGTGAGACC |
| TDF | 55,548,214 | 55,548,236 | GAAGCTCGCTCAGAACTGTAAAC |
| TDP | 55,548,239 | 55,548,263 | TTTCCTCTGCCAGCAGGGATACAGG |
| TDR | 55,548,265 | 55,548,286 | TGGGTTAAGGAAGGGAAGAGTG |
PCR Primers
| Primer | Sequence |
| SU | CCTGGTCTCATCCCTCACTC |
| SD | AACTCAAACACAGAGCAGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13655A-A1 | VGB6 | C57BL/6NTac | |
| 13655A-E3 | VGB6 | C57BL/6NTac | |
| 13655A-G4 | VGB6 | C57BL/6NTac | |
| 13655A-E12 | VGB6 | C57BL/6NTac | |
| 13655A-E10 | VGB6 | C57BL/6NTac | |
| 13655A-D9 | VGB6 | C57BL/6NTac | |
