| Gene | Narg2 |
| Chromosome | 9 |
| Deletion Start | 69,399,050 |
| Deletion End | 69,432,237 |
| Deletion Size | 33,188 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 69,399,066 | 69,399,087 | TGAGTGAACCAAGGCTGAACTG |
| TUP | 69,399,089 | 69,399,116 | TGAGTTGGTCCTCTTCCTGATCCACTGT |
| TUR | 69,399,133 | 69,399,156 | TCCTTTCCTATGATACCCACCATC |
| TDF | 69,432,031 | 69,432,054 | CATCTCTAAGGTTGGTGGGACAAG |
| TDP | 69,432,057 | 69,432,081 | TGCCTACCCGTAACCACAGGAATCC |
| TDR | 69,432,103 | 69,432,122 | GCTGAACAGGCAGGCAACTG |
PCR Primers
| Primer | Sequence |
| SU | TTGGTGGTAAGGGAGTGAGG |
| SD | GGGTCAGAAACAATGCACAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13657A-F12 | VGB6 | C57BL/6NTac | |
| 13657A-B3 | VGB6 | C57BL/6NTac | |
