| Gene | Ndufa2 |
| Chromosome | 18 |
| Deletion Start | 36,744,517 |
| Deletion End | 36,742,560 |
| Deletion Size | 1,958 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 18
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 36,744,251 | 36,744,271 | CGGTACGTGGAGCTGAAGAAG |
| TUP | 36,744,221 | 36,744,244 | CCCAACCTGCCCATTCTGATCCGC |
| TUR | 36,744,202 | 36,744,220 | GCTGCACCTCCGAGCATTC |
| TDF | 36,742,623 | 36,742,645 | GAAGACGGTGTCTCTGAACAATC |
| TDP | 36,742,597 | 36,742,622 | TGAGTGCTGATGAGGTAACCAGAGCC |
| TDR | 36,742,573 | 36,742,593 | TTTGCCGCTTAGCACATTCTG |
PCR Primers
| Primer | Sequence |
| SU | GACTCGAGCCTTACTATCTC |
| SD | CATGCTCTTCAGCAACTTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 70 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13660A-A11 | VGB6 | C57BL/6NTac | |
| 13660A-E12 | VGB6 | C57BL/6NTac | |
| 13660A-E1 | VGB6 | C57BL/6NTac | |
| 13660A-G10 | VGB6 | C57BL/6NTac | |
| 13660A-H8 | VGB6 | C57BL/6NTac | |
