| Gene | Ndufa4l2 |
| Chromosome | 10 |
| Deletion Start | 127,514,985 |
| Deletion End | 127,516,519 |
| Deletion Size | 1,535 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 127,515,186 | 127,515,206 | GTACTCAATGTCCCTGCAACC |
| TUP | 127,515,207 | 127,515,232 | TGCCTTGGACAGGATCACTGTAAGGG |
| TUR | 127,515,249 | 127,515,270 | TGAAACCTACAGCCACATCCTC |
| TDF | 127,516,012 | 127,516,030 | TTGGGTGAGGGAGGCTAAC |
| TDP | 127,516,031 | 127,516,058 | CCTGGGCAGTCTATAGGCTAGGTTGTCA |
| TDR | 127,516,074 | 127,516,095 | GAGGTGCTGGCAAGAAGTATGG |
PCR Primers
| Primer | Sequence |
| SU | TGTCTGCTTGTCTGTCCCAC |
| SD | ACACGCTCAACACGTAGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 85 bp | + | + | - |
| TDF | TDR | 84 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13661A-B2 | VGB6 | C57BL/6NTac | |
| 13661A-B5 | VGB6 | C57BL/6NTac | |
| 13661A-D1 | VGB6 | C57BL/6NTac | |
| 13661A-E3 | VGB6 | C57BL/6NTac | |
| 13661A-H5 | VGB6 | C57BL/6NTac | |
| 13661A-A7 | VGB6 | C57BL/6NTac | |
