| Gene | Nedd8 |
| Chromosome | 14 |
| Deletion Start | 55,671,809 |
| Deletion End | 55,662,522 |
| Deletion Size | 9,288 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 55,671,287 | 55,671,307 | AGTGTCTCACCCGTCTTACAG |
| TUP | 55,671,258 | 55,671,282 | TAAGCTCGGAGTGGTACTAGGCACC |
| TUR | 55,671,213 | 55,671,235 | AGCCAAAGAAATGAGGCTAGTTC |
| TDF | 55,663,337 | 55,663,358 | AAGGTGACGAGGCTACAGGTTC |
| TDP | 55,663,313 | 55,663,336 | ATCGGGCCTGTGCTGCTTCAGTGT |
| TDR | 55,663,285 | 55,663,305 | AGACTGCGAAAGAGCGGCAAG |
PCR Primers
| Primer | Sequence |
| SU | AGTTTGTTCCGTTCCAGCTC |
| SD | TATCCCACCCACACCTTCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 95 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13663A-B2 | VGB6 | C57BL/6NTac | |
| 13663A-C2 | VGB6 | C57BL/6NTac | |
| 13663A-C4 | VGB6 | C57BL/6NTac | |
| 13663A-D5 | VGB6 | C57BL/6NTac | |
| 13663A-D11 | VGB6 | C57BL/6NTac | |
| 13663A-G3 | VGB6 | C57BL/6NTac | |
