| Gene | Neurl2 |
| Chromosome | 2 |
| Deletion Start | 164,833,437 |
| Deletion End | 164,830,804 |
| Deletion Size | 2,634 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 164,832,515 | 164,832,536 | GCCCGCCTGGTATATTCATCTC |
| TUP | 164,832,490 | 164,832,514 | CCCTAGTTGACTGAGACACCCTGCA |
| TUR | 164,832,457 | 164,832,478 | CCCACATCCACATCGTCCAAAC |
| TDF | 164,831,303 | 164,831,324 | GCGAACCTTTCACTTACACCTC |
| TDP | 164,831,278 | 164,831,302 | TGGTCGTGCGTCATGAATTCGTTGA |
| TDR | 164,831,220 | 164,831,244 | CAGCTTTCTGTCTGCTTTGCCTATG |
PCR Primers
| Primer | Sequence |
| SU | TCCGTACCTCTGCTTCCTTG |
| SD | AGATCAGCAGCCCATGTCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 105 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13664A-A11 | VGB6 | C57BL/6NTac | |
| 13664A-B2 | VGB6 | C57BL/6NTac | |
| 13664A-C12 | VGB6 | C57BL/6NTac | |
| 13664A-F7 | VGB6 | C57BL/6NTac | |
| 13664A-F6 | VGB6 | C57BL/6NTac | |
| 13664A-D7 | VGB6 | C57BL/6NTac | |
