| Gene | Ngrn |
| Chromosome | 7 |
| Deletion Start | 80,261,849 |
| Deletion End | 80,265,086 |
| Deletion Size | 3,238 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 80,262,206 | 80,262,228 | ACCTCCAGGACACTGTAGACAAG |
| TUP | 80,262,236 | 80,262,262 | TTGAACTCTTGTCTTCCTGCCTCTGCC |
| TUR | 80,262,290 | 80,262,310 | TCCAGGAGCGTTGGTACATAC |
| TDF | 80,264,773 | 80,264,792 | CCACAGCCTTGGGTCATCAG |
| TDP | 80,264,793 | 80,264,817 | CGAGAGCTTCAGAAATCCGCCACCA |
| TDR | 80,264,848 | 80,264,870 | CACAGCACTTGGTAACGTATCAC |
PCR Primers
| Primer | Sequence |
| SU | CTTCTCATCCGTTGTCTAGG |
| SD | CTGCCGAGAAGAGAACCAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 105 bp | + | + | - |
| TDF | TDR | 98 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13667A-F3 | VGB6 | C57BL/6NTac | |
| 13667A-F6 | VGB6 | C57BL/6NTac | |
