| Gene | Nicn1 |
| Chromosome | 9 |
| Deletion Start | 108,290,534 |
| Deletion End | 108,295,126 |
| Deletion Size | 4,593 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 108,291,033 | 108,291,057 | ACTTGTTTCCAATCCCTAGTTGTTC |
| TUP | 108,291,060 | 108,291,086 | CCATTTCAACTGACAGTGACAGGGCGT |
| TUR | 108,291,100 | 108,291,120 | AGAGGTAGCCTTTGTCCTGTG |
| TDF | 108,294,895 | 108,294,914 | CCAGATCCCAGCAGAGTGTC |
| TDP | 108,294,925 | 108,294,953 | CAGCAGATGTGGGCACTGACAGAGATGAT |
| TDR | 108,294,962 | 108,294,981 | GATCCTCGTGGAAGTGTGAC |
PCR Primers
| Primer | Sequence |
| SU | GGGATAAGACAGCCCAAAGC |
| SD | AACAACAGTGGCTTCCTTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 87 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13669A-D2 | VGB6 | C57BL/6NTac | |
| 13669A-D4 | VGB6 | C57BL/6NTac | |
| 13669A-G4 | VGB6 | C57BL/6NTac | |
| 13669A-E4 | VGB6 | C57BL/6NTac | |
| 13669A-F5 | VGB6 | C57BL/6NTac | |
| 13669A-D6 | VGB6 | C57BL/6NTac | |
