| Gene | Nip7 |
| Chromosome | 8 |
| Deletion Start | 107,056,938 |
| Deletion End | 107,058,560 |
| Deletion Size | 1,623 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 107,057,581 | 107,057,601 | GAGCTGCATAGCCTAGTTGTC |
| TUP | 107,057,609 | 107,057,633 | AAACAGGCCTCCACCGTGTCAGCTG |
| TUR | 107,057,639 | 107,057,660 | GTGTCTTCGTCTCTGCTTTCTG |
| TDF | 107,058,196 | 107,058,215 | CCACGGTAGCCATCCATCAC |
| TDP | 107,058,218 | 107,058,244 | CAAATGCCAGGTACCCACTGGAAAGGA |
| TDR | 107,058,269 | 107,058,290 | GAAAGGAGCCAAGAAGTCAGTC |
PCR Primers
| Primer | Sequence |
| SU | TCTCACGCCACTGAGCTTC |
| SD | AGGCTCATATGGCCGTTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 95 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13670A-G12 | VGB6 | C57BL/6NTac | |
| 13670A-H6 | VGB6 | C57BL/6NTac | |
