| Gene | Nkx6-3 |
| Chromosome | 8 |
| Deletion Start | 23,153,588 |
| Deletion End | 23,157,996 |
| Deletion Size | 4,409 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 23,154,049 | 23,154,067 | TGCACGCGACCTACCTAAC |
| TUP | 23,154,068 | 23,154,092 | CTCAGCCGCTATTACACAGCCCTGC |
| TUR | 23,154,170 | 23,154,189 | ACTGTGGGTCCAGACACCTG |
| TDF | 23,157,567 | 23,157,586 | GGTTGCTCTTTGCAGGTGTG |
| TDP | 23,157,588 | 23,157,609 | TTCCAGAACCGCCGGACCAAGT |
| TDR | 23,157,614 | 23,157,631 | TCCAGGGCGCTCTTCTTC |
PCR Primers
| Primer | Sequence |
| SU | GGTGCTCTGAGTATGGAAAG |
| SD | TAGAAAGCACATCTGGACTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 141 bp | + | + | - |
| TDF | TDR | 65 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13671A-F5 | VGB6 | C57BL/6NTac | |
| 13671A-D5 | VGB6 | C57BL/6NTac | |
| 13671A-G3 | VGB6 | C57BL/6NTac | |
| 13671A-E9 | VGB6 | C57BL/6NTac | |
| 13671A-H6 | VGB6 | C57BL/6NTac | |
