| Gene | Nme2 |
| Chromosome | 11 |
| Deletion Start | 93,955,617 |
| Deletion End | 93,949,895 |
| Deletion Size | 5,723 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 93,955,341 | 93,955,359 | TTTGACCTCCGCCTCTCAC |
| TUP | 93,955,314 | 93,955,334 | TTGGTGGCTCGCTGGCCCTTT |
| TUR | 93,955,251 | 93,955,271 | CGACACCAGTCACGTTACCTC |
| TDF | 93,950,497 | 93,950,518 | AGCTGTCTCTTTAGTCCCATCC |
| TDP | 93,950,456 | 93,950,480 | AATGGTTTACTATTTGCCTTACTGA |
| TDR | 93,950,394 | 93,950,415 | GACCTTAGGACTGACACAGAAC |
PCR Primers
| Primer | Sequence |
| SU | GGCTGGTGGTCGTTCTATTC |
| SD | GCTGGGAGTACCTTAGCTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 109 bp | + | + | - |
| TDF | TDR | 125 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13672B-G2 | VGB6 | C57BL/6NTac | |
| 13672A-H12 | VGB6 | C57BL/6NTac | |
