| Gene | Nme6 |
| Chromosome | 9 |
| Deletion Start | 109,835,328 |
| Deletion End | 109,842,687 |
| Deletion Size | 7,360 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 109,835,348 | 109,835,367 | CCAAGCTCTTCAGCTCACAC |
| TUP | 109,835,369 | 109,835,393 | AGCCCTGATCAAGCCTGATGCAGTT |
| TUR | 109,835,419 | 109,835,438 | TGGAGACTTCGGGTTCATTC |
| TDF | 109,842,598 | 109,842,618 | GGAGGCCACAAACAACCTAAC |
| TDP | 109,842,623 | 109,842,650 | CCTAGGAGACCCAGCCAAGTAAAGTCCC |
| TDR | 109,842,665 | 109,842,682 | GGAGGAAGGGCTGAACAC |
PCR Primers
| Primer | Sequence |
| SU | CTCTTCAGTGGAAAGCTCCC |
| SD | TGCTACACAATCAACCTGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13674A-B9 | VGB6 | C57BL/6NTac | |
| 13674A-E7 | VGB6 | C57BL/6NTac | |
