| Gene | Nop10 |
| Chromosome | 2 |
| Deletion Start | 112,262,027 |
| Deletion End | 112,262,477 |
| Deletion Size | 451 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 112,262,124 | 112,262,145 | AGATGGGAGGAACAGTTGGAAC |
| TUP | 112,262,155 | 112,262,178 | CAGCCCACTGAGTGGAGCCATGTC |
| TUR | 112,262,190 | 112,262,211 | GAAGCCACTGCCTATCTACCAC |
| TDF | 112,262,323 | 112,262,345 | CTATGGGACAACAGACTTGCTCC |
| TDP | 112,262,351 | 112,262,369 | TCCTGCTCGGTTCTCCCCA |
| TDR | 112,262,430 | 112,262,447 | GACAGGACGCGGTTGCTG |
PCR Primers
| Primer | Sequence |
| SU | CAACCCACATCCATCCCTAC |
| SD | AAGGGCCCACAATTAGACAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 125 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13675A-C4 | VGB6 | C57BL/6NTac | |
| 13675A-C10 | VGB6 | C57BL/6NTac | |
| 13675A-D10 | VGB6 | C57BL/6NTac | |
| 13675A-G8 | VGB6 | C57BL/6NTac | |
| 13675A-A2 | VGB6 | C57BL/6NTac | |
| 13675A-F8 | VGB6 | C57BL/6NTac | |
