| Gene | Npff |
| Chromosome | 15 |
| Deletion Start | 102,524,605 |
| Deletion End | 102,523,828 |
| Deletion Size | 778 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 102,524,376 | 102,524,397 | TGGCTTCTAAGGGAGGGAAATG |
| TUP | 102,524,351 | 102,524,373 | TTCTCAGCCCACGCAAGTTTCCC |
| TUR | 102,524,316 | 102,524,338 | ACACTGAAGACATGGAGGGAAAG |
| TDF | 102,523,971 | 102,523,991 | AGCTAAATCCGCAGGCCAGAC |
| TDP | 102,523,948 | 102,523,970 | AGTTCTGGAGCCTGGCCGCTCCT |
| TDR | 102,523,919 | 102,523,942 | TGACGATGCTACTTCTTCCCAAAG |
PCR Primers
| Primer | Sequence |
| SU | ATGCCCAATTCTGACAGGAG |
| SD | GAGACTGAGGAAGGCACAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13677A-A4 | VGB6 | C57BL/6NTac | |
| 13677A-C3 | VGB6 | C57BL/6NTac | |
| 13677A-F10 | VGB6 | C57BL/6NTac | |
| 13677A-D11 | VGB6 | C57BL/6NTac | |
| 13677A-A9 | VGB6 | C57BL/6NTac | |
