| Gene | Npm3 |
| Chromosome | 19 |
| Deletion Start | 45,749,553 |
| Deletion End | 45,748,098 |
| Deletion Size | 1,456 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 45,748,914 | 45,748,932 | ACGCCTTGGCTTTGGTGTC |
| TUP | 45,748,888 | 45,748,913 | AGCCCTCCCTCACTTGAGACGCTGAG |
| TUR | 45,748,869 | 45,748,887 | GCTGAAAGGCAGGCAGAAC |
| TDF | 45,748,458 | 45,748,480 | CTCAGGCTATGGTTCCAGTATTC |
| TDP | 45,748,436 | 45,748,457 | TGTGCCTCCCACTGCCTCCTTC |
| TDR | 45,748,407 | 45,748,427 | CAGGCAGGGTACAGCAAGTTC |
PCR Primers
| Primer | Sequence |
| SU | CTCCAGCATGATCAGGAAGC |
| SD | AAACAACCAACCGTACACCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 64 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13678B-F8 | VGB6 | C57BL/6NTac | |
| 13678B-F5 | VGB6 | C57BL/6NTac | |
| 13678C-B4 | VGB6 | C57BL/6NTac | |
| 13678C-B6 | VGB6 | C57BL/6NTac | |
| 13678B-D6 | VGB6 | C57BL/6NTac | |
| 13678B-F1 | VGB6 | C57BL/6NTac | |
| 13678C-H5 | VGB6 | C57BL/6NTac | |
