| Gene | Nrbp2 |
| Chromosome | 15 |
| Deletion Start | 76,089,621 |
| Deletion End | 76,086,978 |
| Deletion Size | 2,644 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 76,089,530 | 76,089,548 | TTGTCCCTGCACCCAGATG |
| TUP | 76,089,503 | 76,089,527 | TGTACTCGAGATCCAAGCCAACGGG |
| TUR | 76,089,470 | 76,089,487 | TCGAGCGATGGCCTCTTC |
| TDF | 76,087,150 | 76,087,170 | CATGCTTCCCTACTCATCCAC |
| TDP | 76,087,124 | 76,087,149 | TGCAGGATGACAGGACAAAGCTAGCA |
| TDR | 76,087,083 | 76,087,100 | TGCGTCCCTCGGTACTTG |
PCR Primers
| Primer | Sequence |
| SU | GTCTTTCTCTCTGCGTTGGC |
| SD | TGGAAGATCAGAAAGAGGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13682A-B4 | VGB6 | C57BL/6NTac | |
| 13682A-F3 | VGB6 | C57BL/6NTac | |
| 13682A-F7 | VGB6 | C57BL/6NTac | |
| 13682A-F8 | VGB6 | C57BL/6NTac | |
| 13682A-G8 | VGB6 | C57BL/6NTac | |
