13685   Nt5c     Status: ES cell colonies screened / QC positives

GeneNt5c
Chromosome11
Deletion Start115,491,783
Deletion End115,490,507
Deletion Size1,277
Genome Build38
MGI IDMGI:1354954
NCBI Gene ID50773
BAC ID400H22
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 11

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF115,491,634115,491,652CTTCCTCGCCAACGAGCAG
TUP115,491,593115,491,614ACCTGGCGGTAGGAGACCCGAT
TUR115,491,539115,491,557TGTGGATTCGCACAGCTTG
TDF115,490,992115,491,010AGCAAGTTCCACTGACCTC
TDP115,490,961115,490,984CCCTCCCGCCTATACAGTACCGCT
TDR115,490,917115,490,939GATAATCCGCTCCACAAACTCTG

PCR Primers

PrimerSequence
SUTTCTCATCCCTTGAACTGGG
SDGTTCCTGACCACAAAGGTCC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR114 bp++-
TDFTDR94 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
13685A-G5VGB6C57BL/6NTac
13685A-C7VGB6C57BL/6NTac
13685A-B9VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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