| Gene | Nt5dc2 |
| Chromosome | 14 |
| Deletion Start | 31,135,350 |
| Deletion End | 31,139,122 |
| Deletion Size | 3,773 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 31,135,973 | 31,135,995 | ACATGGGTAAGGGTGGACAGTAG |
| TUP | 31,135,998 | 31,136,020 | CTTACCGTGGCCTGCCTGAGCAT |
| TUR | 31,136,039 | 31,136,059 | AGGGAAGATGGGTCTGCAAGG |
| TDF | 31,138,817 | 31,138,836 | GAGCATCTTCCGCACCTTCC |
| TDP | 31,138,839 | 31,138,864 | AACCCTACCTACTTCTCCCGGCGCCT |
| TDR | 31,138,881 | 31,138,902 | AGCTGAGTGAGGCCATGTAGAG |
PCR Primers
| Primer | Sequence |
| SU | CCGGGACATCTTGATAGAGC |
| SD | CAGGTTTCAGCTTCTCTGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 87 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13686A-D7 | VGB6 | C57BL/6NTac | |
| 13686A-D9 | VGB6 | C57BL/6NTac | |
| 13686A-G10 | VGB6 | C57BL/6NTac | |
