| Gene | Nudt19 |
| Chromosome | 7 |
| Deletion Start | 35,555,801 |
| Deletion End | 35,547,820 |
| Deletion Size | 7,982 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 35,555,110 | 35,555,128 | GGGATGCGCTGAGCCTTAC |
| TUP | 35,555,071 | 35,555,094 | CACGTGCGTGACCTTGCTTTAGGC |
| TUR | 35,555,048 | 35,555,069 | GGGTGAGAGTTGAGTCCTTGAG |
| TDF | 35,548,646 | 35,548,667 | GAGGTGGACACAAGCAGATTTC |
| TDP | 35,548,622 | 35,548,645 | TGGATGCTCACAGGCCAAGTATCC |
| TDR | 35,548,596 | 35,548,617 | GGCCTGGAACTTTACCACTTAG |
PCR Primers
| Primer | Sequence |
| SU | ACCTCCTATACAGGCAACAG |
| SD | GGAATCAATTCCTGCGAAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13687A-B6 | VGB6 | C57BL/6NTac | |
| 13687A-B8 | VGB6 | C57BL/6NTac | |
| 13687A-C9 | VGB6 | C57BL/6NTac | |
| 13687A-G3 | VGB6 | C57BL/6NTac | |
| 13687A-H8 | VGB6 | C57BL/6NTac | |
| 13687A-H9 | VGB6 | C57BL/6NTac | |
