| Gene | Nudt22 |
| Chromosome | 19 |
| Deletion Start | 6,995,715 |
| Deletion End | 6,992,947 |
| Deletion Size | 2,769 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 6,994,866 | 6,994,885 | GTTAGGGATTGCCAGGACTC |
| TUP | 6,994,842 | 6,994,865 | TCACACTGAGTCTGCTCCAGGCCT |
| TUR | 6,994,797 | 6,994,816 | TCTATGCCACTTCCCACACC |
| TDF | 6,993,736 | 6,993,753 | CTGCCACTGCACACCTTG |
| TDP | 6,993,710 | 6,993,733 | CCAGCCACTGTTGTTGGGAATCGC |
| TDR | 6,993,654 | 6,993,675 | CCACTCACTGGACATAGAACTC |
PCR Primers
| Primer | Sequence |
| SU | TCCTGGGAGTAGATCAAGCG |
| SD | CATTTCCTGCGCCTATGAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 89 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13688A-A10 | VGB6 | C57BL/6NTac | |
| 13688A-A11 | VGB6 | C57BL/6NTac | |
| 13688A-B12 | VGB6 | C57BL/6NTac | |
| 13688A-C5 | VGB6 | C57BL/6NTac | |
| 13688A-E3 | VGB6 | C57BL/6NTac | |
