| Gene | Nudt4 |
| Chromosome | 10 |
| Deletion Start | 95,563,754 |
| Deletion End | 95,549,258 |
| Deletion Size | 14,497 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 95,563,047 | 95,563,070 | GCGTTCTGAGTTCTGCTTCTGTAG |
| TUP | 95,563,026 | 95,563,045 | CGCGTCCAGGGAAGCACCAA |
| TUR | 95,562,943 | 95,562,964 | CACTGAGTGTACGCTGTCATAC |
| TDF | 95,549,377 | 95,549,396 | GGGCTGTTCTCCGACCAATG |
| TDP | 95,549,351 | 95,549,375 | AAATTCCTCGGTCCCTTCCCTCCCA |
| TDR | 95,549,319 | 95,549,336 | GGCGCAGCAGTCACAAAC |
PCR Primers
| Primer | Sequence |
| SU | ACCGTCGCTTCCAACTGTC |
| SD | TACCCAATAACCAGTTGATCTGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 128 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13689A-B5 | VGB6 | C57BL/6NTac | |
| 13689A-E7 | VGB6 | C57BL/6NTac | |
| 13689A-D7 | VGB6 | C57BL/6NTac | |
| 13689A-E4 | VGB6 | C57BL/6NTac | |
| 13689A-B1 | VGB6 | C57BL/6NTac | |
