| Gene | Nudt6 |
| Chromosome | 3 |
| Deletion Start | 37,419,567 |
| Deletion End | 37,405,096 |
| Deletion Size | 14,472 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 37,419,282 | 37,419,302 | GTGTGGGCTCTGAGGTGAAAG |
| TUP | 37,419,257 | 37,419,280 | TGAAAGGACACCCAGCGGTCCTCT |
| TUR | 37,419,177 | 37,419,196 | GCGAATCCCGTGTCAGTCTC |
| TDF | 37,405,424 | 37,405,443 | TCAGGTCCCTGTTGAGCATC |
| TDP | 37,405,403 | 37,405,423 | CGGCAGCAACACAGGAGCCCT |
| TDR | 37,405,322 | 37,405,344 | TTCCTGCTGGCAGAAGTTGATAG |
PCR Primers
| Primer | Sequence |
| SU | TCAGCTCCACTAAGCTCTAC |
| SD | GACACTTAACCATTACCTCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 126 bp | + | + | - |
| TDF | TDR | 122 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13690A-H9 | VGB6 | C57BL/6NTac | |
| 13690A-E1 | VGB6 | C57BL/6NTac | |
| 13690A-H8 | VGB6 | C57BL/6NTac | |
| 13690A-A6 | VGB6 | C57BL/6NTac | |
