| Gene | Nudt8 |
| Chromosome | 19 |
| Deletion Start | 4,000,657 |
| Deletion End | 4,002,027 |
| Deletion Size | 1,371 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 4,001,147 | 4,001,166 | AACTGCCTCCTGACGACCTG |
| TUP | 4,001,167 | 4,001,189 | TGGTCCCAAGATTCCCTCTGCCC |
| TUR | 4,001,195 | 4,001,213 | CACTTACCGCCTGGGAAAC |
| TDF | 4,001,539 | 4,001,560 | CACCATGATTACCCAAGACACC |
| TDP | 4,001,564 | 4,001,589 | ATGGCAGGGTAGGGTCTGCTTCCTAA |
| TDR | 4,001,590 | 4,001,611 | TGAAGAGTCAGAAGGAGCAAAC |
PCR Primers
| Primer | Sequence |
| SU | TCTCCGAGGATCTGCATCAG |
| SD | CATGGCTCGCTTTCCACAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 67 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13691A-H6 | VGB6 | C57BL/6NTac | |
| 13691A-A11 | VGB6 | C57BL/6NTac | |
| 13691A-B11 | VGB6 | C57BL/6NTac | |
| 13691A-C4 | VGB6 | C57BL/6NTac | |
| 13691A-D10 | VGB6 | C57BL/6NTac | |
| 13691A-A1 | VGB6 | C57BL/6NTac | |
