| Gene | Odf3 |
| Chromosome | 7 |
| Deletion Start | 140,848,358 |
| Deletion End | 140,850,817 |
| Deletion Size | 2,460 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 140,849,160 | 140,849,179 | TGTCGTTCCTGCACACCTTC |
| TUP | 140,849,180 | 140,849,209 | CCTCCTCTCCAGGTGATTACTTTCCAGAGA |
| TUR | 140,849,220 | 140,849,241 | GGGTGCTGAGTCAAACACATAC |
| TDF | 140,850,211 | 140,850,232 | GGTCTGAGCCCTCTTCATCTTC |
| TDP | 140,850,233 | 140,850,253 | CAGACTCCAGGTCCTGCGGCA |
| TDR | 140,850,272 | 140,850,293 | GAGCCTTGAACTTGGTCACTTG |
PCR Primers
| Primer | Sequence |
| SU | GCCAGCTCTTTGGAAAGTTG |
| SD | GCAGCCTGGGTGTAGAGAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13696A-B4 | VGB6 | C57BL/6NTac | |
| 13696A-G12 | VGB6 | C57BL/6NTac | |
| 13696A-D10 | VGB6 | C57BL/6NTac | |
| 13696A-F2 | VGB6 | C57BL/6NTac | |
