| Gene | Cdk16 |
| Chromosome | X |
| Deletion Start | 20,693,333 |
| Deletion End | 20,698,656 |
| Deletion Size | 5,324 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 20,693,836 | 20,693,857 | AGCTACCATCTCAGCTCATCTC |
| TUP | 20,693,867 | 20,693,893 | ACTCCACTAGGTGACCACATTCCCTCC |
| TUR | 20,693,917 | 20,693,937 | CCCTAGAGCAGACTGGGAAAC |
| TDF | 20,698,574 | 20,698,593 | CCACTCACAGCCATTCACTC |
| TDP | 20,698,603 | 20,698,623 | CTGCAGGCAGGCCAGCTTTCC |
| TDR | 20,698,624 | 20,698,644 | AGAACTCGGTATCCACCACAC |
PCR Primers
| Primer | Sequence |
| SU | GGAACATGCCATTCTATGGG |
| SD | AGACCCAAGGCATCACTCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 102 bp | + | + | - |
| TDF | TDR | 71 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13707A-B12 | VGB6 | C57BL/6NTac | |
| 13707A-F6 | VGB6 | C57BL/6NTac | |
| 13707A-G11 | VGB6 | C57BL/6NTac | |
| 13707A-H5 | VGB6 | C57BL/6NTac | |
| 13707A-H7 | VGB6 | C57BL/6NTac | |
