| Gene | Pdap1 |
| Chromosome | 5 |
| Deletion Start | 145,139,976 |
| Deletion End | 145,130,312 |
| Deletion Size | 9,665 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 145,139,072 | 145,139,095 | GGTGGCAGAATAAATCGCTCTAAG |
| TUP | 145,139,048 | 145,139,069 | CCTGGCTAGCTCCCAGCACAGA |
| TUR | 145,138,988 | 145,139,008 | GTGGGCTCACTGCATTAACTG |
| TDF | 145,130,569 | 145,130,586 | GGGCACAGCAGGAACATC |
| TDP | 145,130,542 | 145,130,561 | CTGGCCCTGTGGCCTCAGCA |
| TDR | 145,130,490 | 145,130,509 | TGCGCTAACACAGAGCCAAG |
PCR Primers
| Primer | Sequence |
| SU | TTCCTCTCACTTCCTGTAGG |
| SD | AATCTGGGCTGGTTGTCATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 108 bp | + | + | - |
| TDF | TDR | 97 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13708A-A5 | VGB6 | C57BL/6NTac | |
| 13708B-H4 | VGB6 | C57BL/6NTac | |
| 13708C-G3 | VGB6 | C57BL/6NTac | |
