13714   Pelo     Status: ES cell colonies screened / QC positives

GenePelo
Chromosome13
Deletion Start115,089,916
Deletion End115,088,474
Deletion Size1,443
Genome Build38
MGI IDMGI:2145154
NCBI Gene ID105083
BAC ID436K14
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 13

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF115,089,323115,089,344CATCCAGCGCCACATAAACTTC
TUP115,089,295115,089,318TTGTAAAGTGCGTTCTGGTGGCCA
TUR115,089,269115,089,288GAACTGCTCTCGCACGAATC
TDF115,088,747115,088,769GCCATAGACACTTTGCTCATCAG
TDP115,088,724115,088,746TGACGAGCTCTTCCGGCATCAGG
TDR115,088,664115,088,682TGCCCGCATTCTCCTTCAC

PCR Primers

PrimerSequence
SUAGGGACCCACAACAGACTTG
SDAATTCGGAAACACCAAGTCG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR76 bp++-
TDFTDR106 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
13714A-A6VGB6C57BL/6NTac
13714A-C6VGB6C57BL/6NTac
13714A-D4VGB6C57BL/6NTac
13714A-C11VGB6C57BL/6NTac
13714A-F2VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
Larger Image

VelociGene Genotyping Strategy

Larger Image