| Gene | Pex12 |
| Chromosome | 11 |
| Deletion Start | 83,298,438 |
| Deletion End | 83,296,004 |
| Deletion Size | 2,435 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 83,297,576 | 83,297,593 | TGCTGAAACTGGCTGGAG |
| TUP | 83,297,551 | 83,297,574 | TCGGTTAGCTCGACTGACAGCTCA |
| TUR | 83,297,529 | 83,297,550 | CTGTTTGATAGCTTGCATGTCC |
| TDF | 83,296,448 | 83,296,469 | CTTTGCCTCTTGCTCTCCTTTC |
| TDP | 83,296,417 | 83,296,446 | CAACAGCGTTGGTGAGAAGATAAAGTCAGC |
| TDR | 83,296,391 | 83,296,413 | CAACACCTCCCACAGCTTTCTTC |
PCR Primers
| Primer | Sequence |
| SU | GAGGTGCAGCATTTGACAAG |
| SD | CTGCTTCCCAAACCACTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 65 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13716A-E8 | VGB6 | C57BL/6NTac | |
| 13716A-G11 | VGB6 | C57BL/6NTac | |
| 13716A-G10 | VGB6 | C57BL/6NTac | |
