| Gene | Pgam2 |
| Chromosome | 11 |
| Deletion Start | 5,803,685 |
| Deletion End | 5,801,572 |
| Deletion Size | 2,114 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 5,803,497 | 5,803,514 | GTGCTGAAGCGGGCAATC |
| TUP | 5,803,473 | 5,803,496 | CGCACCCTTTGGACCATCCTGGAT |
| TUR | 5,803,446 | 5,803,463 | CACGGGCACCCACATTTG |
| TDF | 5,802,111 | 5,802,130 | AGGAGCAGGCAGTGGGTCAG |
| TDP | 5,802,087 | 5,802,109 | TTTCCTGTTCCTCCCGGGTCAGA |
| TDR | 5,802,024 | 5,802,044 | CCCAGTGTGAGCTGGCTAAAG |
PCR Primers
| Primer | Sequence |
| SU | GGGCCAATTGTGATTGAAAG |
| SD | CTTCTGCCTGTCCTTTAGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 69 bp | + | + | - |
| TDF | TDR | 107 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13717B-G3 | VGB6 | C57BL/6NTac | |
| 13717C-B8 | VGB6 | C57BL/6NTac | |
| 13717C-D12 | VGB6 | C57BL/6NTac | |
| 13717C-E5 | VGB6 | C57BL/6NTac | |
| 13717C-F1 | VGB6 | C57BL/6NTac | |
