| Gene | Phf23 |
| Chromosome | 11 |
| Deletion Start | 69,996,023 |
| Deletion End | 69,999,515 |
| Deletion Size | 3,493 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 69,996,038 | 69,996,058 | GAGCCCAGTCCCGAAGGTAAG |
| TUP | 69,996,062 | 69,996,084 | TCCATAGGCACAGAGGACAGGGA |
| TUR | 69,996,099 | 69,996,119 | GACCTCCAACTTTGCCCATTG |
| TDF | 69,999,046 | 69,999,066 | GCAGCACAGAAACAAGCCAAG |
| TDP | 69,999,071 | 69,999,096 | AGATGCTAGCTCCAGTGAAGGCGAGA |
| TDR | 69,999,133 | 69,999,151 | GGGCAGCCCAACTTCTCAC |
PCR Primers
| Primer | Sequence |
| SU | TTCCGGAGGATCTTGCATAC |
| SD | CTCCACCTCTGAGCAACCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 106 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13719A-A2 | VGB6 | C57BL/6NTac | |
| 13719A-A11 | VGB6 | C57BL/6NTac | |
| 13719A-G6 | VGB6 | C57BL/6NTac | |
| 13719A-G8 | VGB6 | C57BL/6NTac | |
| 13719A-H9 | VGB6 | C57BL/6NTac | |
| 13719A-H11 | VGB6 | C57BL/6NTac | |
