| Gene | Pitx3 |
| Chromosome | 19 |
| Deletion Start | 46,137,479 |
| Deletion End | 46,135,951 |
| Deletion Size | 1,529 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 46,137,427 | 46,137,446 | AGCCCTGCGCTGTCGTTATC |
| TUP | 46,137,399 | 46,137,421 | CAGGCACTCCACACCCTCCGCTT |
| TUR | 46,137,379 | 46,137,397 | CCCTTGCAGCCATGTTCTG |
| TDF | 46,136,687 | 46,136,708 | CCACCATGCACCTCTGATCTTC |
| TDP | 46,136,660 | 46,136,681 | CCGGGCTCGATCCTACTGTGGG |
| TDR | 46,136,605 | 46,136,624 | CCTGCGGGTATCCGAGAAAG |
PCR Primers
| Primer | Sequence |
| SU | TACTCTATTCTATAGTGCACC |
| SD | GAAGGATGAAGGGAAGGAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 68 bp | + | + | - |
| TDF | TDR | 104 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13722A-C12 | VGB6 | C57BL/6NTac | |
| 13722A-A4 | VGB6 | C57BL/6NTac | |
| 13722B-H6 | VGB6 | C57BL/6NTac | |
| 13722B-G12 | VGB6 | C57BL/6NTac | |
| 13722C-H12 | VGB6 | C57BL/6NTac | |
