| Gene | Pkd1l3 |
| Chromosome | 8 |
| Deletion Start | 109,614,520 |
| Deletion End | 109,640,906 |
| Deletion Size | 26,387 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 109,614,755 | 109,614,776 | AAGAAACAGTGTGGTGGGTCAG |
| TUP | 109,614,781 | 109,614,802 | AACCTGACGCTGCCCGGATCGC |
| TUR | 109,614,821 | 109,614,840 | CAACACGAGAGCCCTACCTG |
| TDF | 109,640,594 | 109,640,614 | CAGGAACCTCCCGAGGGATAG |
| TDP | 109,640,616 | 109,640,640 | AGGTGGGCCTGGCTCATCTGCTTCT |
| TDR | 109,640,686 | 109,640,703 | GTCGCCTGGCACTTTCAC |
PCR Primers
| Primer | Sequence |
| SU | GACACAGATGGAGACATTGG |
| SD | AACGGAAGCTTTGCTTCTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 110 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13723A-A1 | VGB6 | C57BL/6NTac | |
| 13723A-D1 | VGB6 | C57BL/6NTac | |
| 13723A-B1 | VGB6 | C57BL/6NTac | |
| 13723A-C7 | VGB6 | C57BL/6NTac | |
| 13723A-A7 | VGB6 | C57BL/6NTac | |
| 13723A-G5 | VGB6 | C57BL/6NTac | |
