| Gene | Plagl2 |
| Chromosome | 2 |
| Deletion Start | 153,236,056 |
| Deletion End | 153,231,406 |
| Deletion Size | 4,651 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 153,235,772 | 153,235,794 | TGACACTCCCATTCCTCTTTCAG |
| TUP | 153,235,746 | 153,235,770 | TTCCAGGTTCCTGGAGCTCACAACC |
| TUR | 153,235,707 | 153,235,729 | CACAGGACAACTTTACCGTTCAC |
| TDF | 153,232,274 | 153,232,293 | CCGCAAGGATCACCTGACAC |
| TDP | 153,232,246 | 153,232,272 | CCATGTCAAGAAGAGCCACTCACAGGA |
| TDR | 153,232,199 | 153,232,222 | TGAGCAAGCCTAACATATCCACTG |
PCR Primers
| Primer | Sequence |
| SU | TTGGCATCTTTATTGCATGG |
| SD | AGGGACTCAACAGCCAACTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 95 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13724A-A6 | VGB6 | C57BL/6NTac | |
| 13724A-C2 | VGB6 | C57BL/6NTac | |
| 13724A-C3 | VGB6 | C57BL/6NTac | |
| 13724A-D5 | VGB6 | C57BL/6NTac | |
| 13724A-D7 | VGB6 | C57BL/6NTac | |
| 13724A-E9 | VGB6 | C57BL/6NTac | |
