| Gene | Pold2 |
| Chromosome | 11 |
| Deletion Start | 5,876,929 |
| Deletion End | 5,872,158 |
| Deletion Size | 4,772 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 5,876,831 | 5,876,851 | CCTGTGGCAACTTACACCAAC |
| TUP | 5,876,807 | 5,876,828 | CTCACAGCCCTTCCGACTGGGA |
| TUR | 5,876,735 | 5,876,755 | CACCAGGAACGGTCTCATCTG |
| TDF | 5,872,929 | 5,872,947 | TGGCAACCACCCTGGAATG |
| TDP | 5,872,905 | 5,872,927 | TGATTCCGTTCATGGCCAGTGCC |
| TDR | 5,872,860 | 5,872,884 | GCTCTGTGGAAGTAGGAGCTGTATG |
PCR Primers
| Primer | Sequence |
| SU | CTTCAGTGATGCCTGATAGC |
| SD | TGCCCAATTCAAACTAAGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 117 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13729A-A7 | VGB6 | C57BL/6NTac | |
| 13729A-H11 | VGB6 | C57BL/6NTac | |
| 13729A-C7 | VGB6 | C57BL/6NTac | |
| 13729A-C1 | VGB6 | C57BL/6NTac | |
| 13729A-G3 | VGB6 | C57BL/6NTac | |
