| Gene | Ppp1r15b |
| Chromosome | 1 |
| Deletion Start | 133,131,750 |
| Deletion End | 133,136,900 |
| Deletion Size | 5,151 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 133,132,275 | 133,132,294 | GTGGAGTTGCTGCCCAGTAG |
| TUP | 133,132,295 | 133,132,321 | CCTTCAAGCTGGTCTAGTCTCCCACCG |
| TUR | 133,132,331 | 133,132,350 | GAGGCCCAGAGGATGAAGAG |
| TDF | 133,136,702 | 133,136,721 | GGTGATGAGGATCGCAAAGG |
| TDP | 133,136,730 | 133,136,759 | AAGAATTTGCAAGGGATGGATGCAGGTTCC |
| TDR | 133,136,788 | 133,136,806 | GGCCAAGCAGTAGCCAATG |
PCR Primers
| Primer | Sequence |
| SU | TTTGAGCTTCGCTTCTTTGC |
| SD | ACCCACATCCAACTAAATAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 105 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13740A-E1 | VGB6 | C57BL/6NTac | |
| 13740A-B9 | VGB6 | C57BL/6NTac | |
