| Gene | Pramel6 |
| Chromosome | 2 |
| Deletion Start | 87,508,461 |
| Deletion End | 87,510,814 |
| Deletion Size | 2,354 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 87,509,271 | 87,509,292 | AGCACAGATCGTGGCACAGAAG |
| TUP | 87,509,296 | 87,509,318 | CCAGTGGAGACCTGCCCTAACCC |
| TUR | 87,509,350 | 87,509,369 | TCCTGCGTGAGTTCAAAGTC |
| TDF | 87,510,396 | 87,510,415 | TCTGGAACTGGAGAGGTGTG |
| TDP | 87,510,419 | 87,510,445 | TGACAGACCCTCATTTCAAAGCCCTCA |
| TDR | 87,510,459 | 87,510,482 | ACTTTAAGGAGCTGGGAGCATTTG |
PCR Primers
| Primer | Sequence |
| SU | CTCCATTTGTCTCTCCAGGG |
| SD | TCTTTGAGGATGCCACAACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 99 bp | + | + | - |
| TDF | TDR | 87 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13741A-A6 | VGB6 | C57BL/6NTac | |
| 13741A-A9 | VGB6 | C57BL/6NTac | |
| 13741A-C7 | VGB6 | C57BL/6NTac | |
| 13741A-C9 | VGB6 | C57BL/6NTac | |
| 13741A-G11 | VGB6 | C57BL/6NTac | |
| 13741A-F5 | VGB6 | C57BL/6NTac | |
