| Gene | Psmc3ip |
| Chromosome | 11 |
| Deletion Start | 101,095,373 |
| Deletion End | 101,092,330 |
| Deletion Size | 3,044 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 101,095,041 | 101,095,061 | GGGTCGCGCTAATTGTTTCAC |
| TUP | 101,095,016 | 101,095,040 | CCTCTCCTCTGCTAGGCGGTAGTGA |
| TUR | 101,094,959 | 101,094,977 | GCTTGCCGTAGGTCTTCTC |
| TDF | 101,092,808 | 101,092,827 | TGCAGTGAACAGACACAAGG |
| TDP | 101,092,777 | 101,092,804 | TCAGACAAGCACACTCCTTCCTTTCAGG |
| TDR | 101,092,731 | 101,092,752 | TTCCGCTTCCTCCACTCTTTAC |
PCR Primers
| Primer | Sequence |
| SU | TAGTCCCACTCCTGTGGAGG |
| SD | TTGTCTCCATTTCTCCTGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 103 bp | + | + | - |
| TDF | TDR | 97 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13744A-C12 | VGB6 | C57BL/6NTac | |
| 13744A-F7 | VGB6 | C57BL/6NTac | |
| 13744A-D12 | VGB6 | C57BL/6NTac | |
| 13744A-F6 | VGB6 | C57BL/6NTac | |
