| Gene | Pth |
| Chromosome | 7 |
| Deletion Start | 113,386,277 |
| Deletion End | 113,385,762 |
| Deletion Size | 516 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 113,386,223 | 113,386,242 | CATGCTGGCAGTCTGTCTTC |
| TUP | 113,386,198 | 113,386,220 | ACCCAAACGGATGGGAAACCCGT |
| TUR | 113,386,096 | 113,386,114 | AGCGGACCGTTCCATTAGC |
| TDF | 113,385,978 | 113,386,000 | GAGAAGGAAGCTGCAAGATATGC |
| TDP | 113,385,943 | 113,385,970 | TGTTAGTCTTGGAGTCCAAATGGCTGCC |
| TDR | 113,385,916 | 113,385,934 | GGTGGGCTTCTGGTGACTG |
PCR Primers
| Primer | Sequence |
| SU | AGCTTAGCCATGGAAACAGTG |
| SD | AACTTCTGAGCCAACCCTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 147 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13746A-D10 | VGB6 | C57BL/6NTac | |
| 13746A-H4 | VGB6 | C57BL/6NTac | |
| 13746A-G11 | VGB6 | C57BL/6NTac | |
| 13746A-F3 | VGB6 | C57BL/6NTac | |
| 13746A-E1 | VGB6 | C57BL/6NTac | |
| 13746A-H1 | VGB6 | C57BL/6NTac | |
