| Gene | Pxmp4 |
| Chromosome | 2 |
| Deletion Start | 154,603,626 |
| Deletion End | 154,587,823 |
| Deletion Size | 15,804 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 154,603,371 | 154,603,388 | TGGTGCAGCAGACGTAGG |
| TUP | 154,603,342 | 154,603,367 | CAGGGTTCCAGTCACCTTACCAGTCC |
| TUR | 154,603,286 | 154,603,305 | TGCCAGGTTGGAAAGGACAG |
| TDF | 154,588,116 | 154,588,136 | GAGGCTGTGCTGACGAGTAAC |
| TDP | 154,588,092 | 154,588,115 | CCTGAGCATTTCCCTCCCTGCAGA |
| TDR | 154,587,998 | 154,588,017 | GGGAACGGGTCCCACTTGAG |
PCR Primers
| Primer | Sequence |
| SU | CGAAAGAGAACAGCAAACCC |
| SD | GAAGTCGGAAGAGCATCTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 103 bp | + | + | - |
| TDF | TDR | 139 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13749A-E5 | VGB6 | C57BL/6NTac | |
| 13749A-G3 | VGB6 | C57BL/6NTac | |
| 13749A-E11 | VGB6 | C57BL/6NTac | |
| 13749A-B11 | VGB6 | C57BL/6NTac | |
