| Gene | Qprt |
| Chromosome | 7 |
| Deletion Start | 127,121,982 |
| Deletion End | 127,107,997 |
| Deletion Size | 13,986 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 127,121,687 | 127,121,709 | GATGGACTGGTAGTGGGAGTTTG |
| TUP | 127,121,655 | 127,121,678 | AGCAGCCCTGGACTCTGTCCCTTG |
| TUR | 127,121,610 | 127,121,631 | ACAGGGATGAGACCGGACTTTG |
| TDF | 127,108,639 | 127,108,659 | GCATTTCAGTCCCTCCACCAG |
| TDP | 127,108,614 | 127,108,638 | AGACCTGCCTGGTGACAAGTGGTCC |
| TDR | 127,108,560 | 127,108,582 | AAGACAGCCTCCTCCATCTACAG |
PCR Primers
| Primer | Sequence |
| SU | ATAGGAGAGTGAGGTCCGGC |
| SD | CCCTAGCCACACAGATAGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 100 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13751A-A9 | VGB6 | C57BL/6NTac | |
| 13751A-B11 | VGB6 | C57BL/6NTac | |
| 13751A-D9 | VGB6 | C57BL/6NTac | |
| 13751A-E4 | VGB6 | C57BL/6NTac | |
| 13751A-F9 | VGB6 | C57BL/6NTac | |
| 13751A-H9 | VGB6 | C57BL/6NTac | |
