| Gene | Adcy6 |
| Chromosome | 15 |
| Deletion Start | 98,604,728 |
| Deletion End | 98,592,336 |
| Deletion Size | 12,393 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 98,604,351 | 98,604,372 | TGTGCAGGTGTTCCAGTCTAAG |
| TUP | 98,604,326 | 98,604,349 | AGTTCCGCTCTGCCAAGCTGGAGC |
| TUR | 98,604,282 | 98,604,300 | GAGGCTGCTCTGGTTCATC |
| TDF | 98,592,823 | 98,592,841 | TGGGACATCCTAGCCTCAG |
| TDP | 98,592,789 | 98,592,818 | TTGCTACTGTCTCTACTTCAGGGTTGAACA |
| TDR | 98,592,761 | 98,592,780 | CCAATGACGCCTGCTACAAC |
PCR Primers
| Primer | Sequence |
| SU | TTTATCAAGACGGTGGGAATG |
| SD | AAGTGTTCTTCATGCCTGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13753A-H2 | VGB6 | C57BL/6NTac | |
| 13753B-A11 | VGB6 | C57BL/6NTac | |
| 13753B-B8 | VGB6 | C57BL/6NTac | |
| 13753B-C1 | VGB6 | C57BL/6NTac | |
