| Gene | Rabac1 |
| Chromosome | 7 |
| Deletion Start | 24,972,579 |
| Deletion End | 24,969,919 |
| Deletion Size | 2,661 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 24,971,882 | 24,971,900 | GCAGCCTCGGATACCTAAC |
| TUP | 24,971,856 | 24,971,880 | CCTGTCTCCCACACTCTTCCAGGGT |
| TUR | 24,971,820 | 24,971,839 | AAGACAGCCAGAGCCACCAG |
| TDF | 24,970,202 | 24,970,221 | TGGGAACGGCTACCTTACAC |
| TDP | 24,970,180 | 24,970,201 | CATCTGTTCCCAGGCCGGGAGG |
| TDR | 24,970,148 | 24,970,168 | ACCAGCCAGGGCATACTGATG |
PCR Primers
| Primer | Sequence |
| SU | TGCTGTAGTCCACGCTGAG |
| SD | CCCTCTCCTTACCTATAAAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13756A-D10 | VGB6 | C57BL/6NTac | |
| 13756A-E4 | VGB6 | C57BL/6NTac | |
| 13756A-E8 | VGB6 | C57BL/6NTac | |
| 13756A-H3 | VGB6 | C57BL/6NTac | |
| 13756A-G3 | VGB6 | C57BL/6NTac | |
| 13756A-E1 | VGB6 | C57BL/6NTac | |
