| Gene | Rabggta |
| Chromosome | 14 |
| Deletion Start | 55,721,843 |
| Deletion End | 55,716,312 |
| Deletion Size | 5,532 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 55,721,664 | 55,721,682 | GTCCGAAGAGCAGGCAGAG |
| TUP | 55,721,630 | 55,721,658 | AAGGCTAGAGCGGGAACAGAAGCTAAAGC |
| TUR | 55,721,608 | 55,721,628 | GCTTGAGTGGCTGACTGGTAG |
| TDF | 55,716,477 | 55,716,497 | CCCAGGCTAGTCTTCCTCAAC |
| TDP | 55,716,456 | 55,716,475 | TGCAGGGCAACTCGCTGTGC |
| TDR | 55,716,413 | 55,716,433 | ACGGTAGCATCTCAGCCAAAC |
PCR Primers
| Primer | Sequence |
| SU | CTCCCTGAGCAGGTGAGTTC |
| SD | TGAGCAGTCCAAACCACAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13758A-H8 | VGB6 | C57BL/6NTac | |
| 13758A-E2 | VGB6 | C57BL/6NTac | |
| 13758A-E8 | VGB6 | C57BL/6NTac | |
| 13758A-G3 | VGB6 | C57BL/6NTac | |
| 13758A-G10 | VGB6 | C57BL/6NTac | |
| 13758A-F3 | VGB6 | C57BL/6NTac | |
