| Gene | Slc50a1 |
| Chromosome | 3 |
| Deletion Start | 89,270,462 |
| Deletion End | 89,268,455 |
| Deletion Size | 2,008 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 89,270,013 | 89,270,034 | AAGGCTGAGGGAGGTGCAAGAG |
| TUP | 89,269,989 | 89,270,012 | ATCCCTGGAGACCTGCTGCAACCC |
| TUR | 89,269,951 | 89,269,975 | CTGTGAGGGAAGGAGGATTAAGGTC |
| TDF | 89,268,776 | 89,268,796 | GTAGGCCAAGATCGTTCAGAC |
| TDP | 89,268,751 | 89,268,774 | AAATCAACCCAGCGCCTCTCCTTC |
| TDR | 89,268,690 | 89,268,712 | GGAGGCGAAACCCGTAAATAGAC |
PCR Primers
| Primer | Sequence |
| SU | GCTGGGTAACCAAGGTTCTG |
| SD | TGTCTGTCAAGTCAGGCAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 107 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13759A-G7 | VGB6 | C57BL/6NTac | |
| 13759A-B7 | VGB6 | C57BL/6NTac | |
| 13759A-E10 | VGB6 | C57BL/6NTac | |
| 13759A-A4 | VGB6 | C57BL/6NTac | |
