| Gene | Ramp2 |
| Chromosome | 11 |
| Deletion Start | 101,246,455 |
| Deletion End | 101,248,160 |
| Deletion Size | 1,706 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 101,246,655 | 101,246,674 | CCACCCTGCGAGTACCAATC |
| TUP | 101,246,677 | 101,246,699 | CGTGCGGAGCACCTCTGAACTGC |
| TUR | 101,246,709 | 101,246,731 | CGCCAACCCAGAGATGTATTCAG |
| TDF | 101,247,693 | 101,247,714 | GGGCAGTGTTGAAGGATGGATG |
| TDP | 101,247,717 | 101,247,740 | TCAGGCCAGGAGTTGGACCTGTTC |
| TDR | 101,247,759 | 101,247,778 | TGGATGGGCACAGGGAGAAG |
PCR Primers
| Primer | Sequence |
| SU | CCCTGCCTCAGATACCCAG |
| SD | TCCCACACCTTCCCAACTAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13760A-H8 | VGB6 | C57BL/6NTac | |
| 13760A-A6 | VGB6 | C57BL/6NTac | |
