| Gene | Esrp2 |
| Chromosome | 8 |
| Deletion Start | 106,136,764 |
| Deletion End | 106,131,417 |
| Deletion Size | 5,348 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 106,136,168 | 106,136,188 | CCATTCCCAGAGCCACCTTAC |
| TUP | 106,136,138 | 106,136,165 | TCCAACCTTTACAGACATCCACTCCTGC |
| TUR | 106,136,115 | 106,136,134 | AGCCGCTATTGTCAACTTCC |
| TDF | 106,132,164 | 106,132,182 | GCTGCTGCCACTCCTCTTG |
| TDP | 106,132,139 | 106,132,162 | CTATTACCCAGGGCCAGCCACTCA |
| TDR | 106,132,105 | 106,132,126 | CCTGGGATAGTAGGCTGTGTAG |
PCR Primers
| Primer | Sequence |
| SU | GCCTCTGATCCTGCACATTC |
| SD | CCAGATATCCTGAGGGCAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 74 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13762A-H9 | VGB6 | C57BL/6NTac | |
| 13762A-B2 | VGB6 | C57BL/6NTac | |
| 13762A-G3 | VGB6 | C57BL/6NTac | |
| 13762A-F3 | VGB6 | C57BL/6NTac | |
| 13762A-F10 | VGB6 | C57BL/6NTac | |
| 13762A-F9 | VGB6 | C57BL/6NTac | |
