| Gene | Rbm8a |
| Chromosome | 3 |
| Deletion Start | 96,629,965 |
| Deletion End | 96,631,772 |
| Deletion Size | 1,808 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 96,630,511 | 96,630,529 | CGACAGTGTGGAGCAGGAC |
| TUP | 96,630,532 | 96,630,554 | CGATGAACCTGGACCACAGCGCT |
| TUR | 96,630,570 | 96,630,589 | TGCACACAGACTCCCCATAG |
| TDF | 96,631,512 | 96,631,530 | GCAGCCAATCAGTGTGGAC |
| TDP | 96,631,531 | 96,631,554 | TGGTGTTTTGTTCGTGGACCACCA |
| TDR | 96,631,559 | 96,631,581 | CCCTTCATCTTACCTCCTCTTGC |
PCR Primers
| Primer | Sequence |
| SU | GGCTGTAGAACTTCCGAAACC |
| SD | AGGCTAAGCATCACAAAGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 70 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13764A-D5 | VGB6 | C57BL/6NTac | |
| 13764A-A12 | VGB6 | C57BL/6NTac | |
