| Gene | Rfx5 |
| Chromosome | 3 |
| Deletion Start | 94,955,037 |
| Deletion End | 94,959,444 |
| Deletion Size | 4,408 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 94,955,196 | 94,955,214 | TCCTTGTTGCCCTGCACTC |
| TUP | 94,955,216 | 94,955,240 | TTCCTCAGGCAAGTGTTTCAGAGCA |
| TUR | 94,955,254 | 94,955,274 | CCAAACAGCTACGGACCACAG |
| TDF | 94,959,088 | 94,959,107 | AGGCTGAGCGGGAAACAGTG |
| TDP | 94,959,108 | 94,959,133 | CTTGTGCAAGGTCAGCAAGATGGTGC |
| TDR | 94,959,158 | 94,959,178 | TCTTTGGCTTCCTGGGAACTC |
PCR Primers
| Primer | Sequence |
| SU | TAACGTGGACTTCTCCACCC |
| SD | GGACTGCCTCTTGATTCTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 91 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13768A-A1 | VGB6 | C57BL/6NTac | |
| 13768A-G8 | VGB6 | C57BL/6NTac | |
| 13768A-E5 | VGB6 | C57BL/6NTac | |
| 13768A-B10 | VGB6 | C57BL/6NTac | |
