| Gene | Rgs12 |
| Chromosome | 5 |
| Deletion Start | 34,964,878 |
| Deletion End | 34,975,329 |
| Deletion Size | 10,452 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 34,965,098 | 34,965,120 | CCCATGAAGATGTGGTGAAACTG |
| TUP | 34,965,123 | 34,965,146 | CGGGAAATGCTCTGGAGTCCTGCA |
| TUR | 34,965,180 | 34,965,197 | TCATCGCTGGAACAGGAC |
| TDF | 34,975,113 | 34,975,131 | ACAGTGCTGCTGGGCTTGG |
| TDP | 34,975,137 | 34,975,164 | CCTTCAGTGGAGCACAAAGGACAGAACA |
| TDR | 34,975,165 | 34,975,185 | CCCTCCTGGGAAGACACTATC |
PCR Primers
| Primer | Sequence |
| SU | CATCAGGGTTCTTAGCCCAG |
| SD | GCCTTTGATGTGGGATGATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 100 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13769A-B4 | VGB6 | C57BL/6NTac | |
| 13769A-D5 | VGB6 | C57BL/6NTac | |
| 13769A-G8 | VGB6 | C57BL/6NTac | |
| 13769A-H11 | VGB6 | C57BL/6NTac | |
| 13769A-D11 | VGB6 | C57BL/6NTac | |
