Information regarding availability, timelines and delivery of materials can be found at www.komp.org or www.knockoutmouse.org.

13771 Rgs2 Status: ES cell colonies screened / QC positives

Gene	Rgs2
Chromosome	1
Deletion Start	144,004,115
Deletion End	144,001,643
Deletion Size	2,473
Genome Build	38

MGI ID	MGI:1098271
NCBI Gene ID	19735
BAC ID	374I17
BAC Library	bMQ
Cassette	ZEN-Ub1
Clones Available	Yes

Sequence Downloads and Strategy Information

Primer Genomic Coordinates on Chromosome 1

Please be aware that these oligonucleotides have been validated only by Taqman assay.

Primer	Start	End	Sequence
TUF	144,003,686	144,003,705	ATAACGTCCACCGGCGTCTG
TUP	144,003,654	144,003,681	TTGCATTGTTAGTTCTGAGCTGCTTGCA
TUR	144,003,615	144,003,638	ACATTGCGAATCAACTCAAACATC
TDF	144,002,108	144,002,131	GGAAGCTCCCAAAGAGGTAAGAAG
TDP	144,002,075	144,002,102	CGCCTCAGATCACCTTCAACACTGTTGA
TDR	144,002,036	144,002,057	TCACTTGTGCTTGAATGTGGTG

PCR Primers

Primer	Sequence
SU	ATCCCAGCTGCAGTTTATGG
SD	AAGAACGTCAACACCCTTGC
LacInR	TTGACTGTAGCGGCTGATGTTG
LacInf	GGTAAACTGGCTCGGATTAGGG
NeoInR	TACTTTCTCGGCAGGAGCAAGGTG
NeoInF	TTCGGCTATGACTGGGCACAACAG
LacInZRev	GTCTGTCCTAGCTTCCTCACTG
NeoFwd	TCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward Primer	Reverse Primer	Length	WT	HET	KO
SU	LacZRev	bp	-	+	+
TUF	TUR	91 bp	+	+	-
TDF	TDR	96 bp	+	+	-
NeoFwd	SD	bp	-	+	+
LacInf	LacInR	210 bp	-	+	+
NeoInf	NeoInR	282 bp	-	+	+

Available Clones

ES Cell ordering information can be found at the KOMP Repository.

Clone Name	Parental ES Cell	Mouse Strain	Cassette Copy Number
13771A-D2	VGB6	C57BL/6NTac
13771A-B4	VGB6	C57BL/6NTac
13771A-E12	VGB6	C57BL/6NTac